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microfluidic plastic chips and chip holders  (AIM Biotech)

 
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    AIM Biotech microfluidic plastic chips and chip holders
    Microfluidic Plastic Chips And Chip Holders, supplied by AIM Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfluidic plastic chips and chip holders/product/AIM Biotech
    Average 90 stars, based on 1 article reviews
    microfluidic plastic chips and chip holders - by Bioz Stars, 2026-03
    90/100 stars

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    miR-184 suppresses growth and migration of human primary choroidal endothelial cells (hCEC) A Schematic of isolation of endothelial cells from fresh donor eyes using CD31( +) magnetic beads and culture and assay of miR-184 mimic and inhibitor transfected cells. B 3D Tube formation assay using miR-184 mimic/inhibitor transfected hCEC (primary human choroidal endothelial cell) at 4 h after seeding. Images were skeletonized and measured for analysis. Scrambled negative controls for mimic and inhibitor are indicated as N.C. C , D Total C loops and D branching points were measured for tube formation property parameters at 4 h. Note a significant decrement of total loops and branching points at miR-184 mimic transfected cells. E Wound healing assay using miR-184 mimic/inhibitor-transfected hCEC. The wound space was measured at 2 h after scratching. F , G Measurement of the wound space area. F miR-184 mimic transfected cells showed reduced wound closure, while G no significant difference was observed in the miR-184 inhibitor transfected cells. H Schematic of sprouting angiogenesis assay using <t>microfluidics</t> chip. 20 ng/mL of VEGF gradient was generated to induce endothelial cell sprouting. I Sprouting angiogenesis assay using miR-184 mimic/inhibitor transfected hCEC. Cells were analyzed at 48 h after generating a VEGF gradient. J The number of tip cells per mm was measured and analyzed. miR-184 mimic transfected cells express significantly low tip cells. All assays were performed with three replicative cultures. Graph values are represented as mean ± standard deviation. Statistical significance indicated as *P < 0.05, **P < 0.01, (all n = 3), B , C Scale bar = 1 mm. E – I Scale bar = 20 μm
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    miR-184 suppresses growth and migration of human primary choroidal endothelial cells (hCEC) A Schematic of isolation of endothelial cells from fresh donor eyes using CD31( +) magnetic beads and culture and assay of miR-184 mimic and inhibitor transfected cells. B 3D Tube formation assay using miR-184 mimic/inhibitor transfected hCEC (primary human choroidal endothelial cell) at 4 h after seeding. Images were skeletonized and measured for analysis. Scrambled negative controls for mimic and inhibitor are indicated as N.C. C , D Total C loops and D branching points were measured for tube formation property parameters at 4 h. Note a significant decrement of total loops and branching points at miR-184 mimic transfected cells. E Wound healing assay using miR-184 mimic/inhibitor-transfected hCEC. The wound space was measured at 2 h after scratching. F , G Measurement of the wound space area. F miR-184 mimic transfected cells showed reduced wound closure, while G no significant difference was observed in the miR-184 inhibitor transfected cells. H Schematic of sprouting angiogenesis assay using microfluidics chip. 20 ng/mL of VEGF gradient was generated to induce endothelial cell sprouting. I Sprouting angiogenesis assay using miR-184 mimic/inhibitor transfected hCEC. Cells were analyzed at 48 h after generating a VEGF gradient. J The number of tip cells per mm was measured and analyzed. miR-184 mimic transfected cells express significantly low tip cells. All assays were performed with three replicative cultures. Graph values are represented as mean ± standard deviation. Statistical significance indicated as *P < 0.05, **P < 0.01, (all n = 3), B , C Scale bar = 1 mm. E – I Scale bar = 20 μm

    Journal: Journal of Nanobiotechnology

    Article Title: Exosomal miR-184 in the aqueous humor of patients with central serous chorioretinopathy: a potential diagnostic and prognostic biomarker

    doi: 10.1186/s12951-023-02019-6

    Figure Lengend Snippet: miR-184 suppresses growth and migration of human primary choroidal endothelial cells (hCEC) A Schematic of isolation of endothelial cells from fresh donor eyes using CD31( +) magnetic beads and culture and assay of miR-184 mimic and inhibitor transfected cells. B 3D Tube formation assay using miR-184 mimic/inhibitor transfected hCEC (primary human choroidal endothelial cell) at 4 h after seeding. Images were skeletonized and measured for analysis. Scrambled negative controls for mimic and inhibitor are indicated as N.C. C , D Total C loops and D branching points were measured for tube formation property parameters at 4 h. Note a significant decrement of total loops and branching points at miR-184 mimic transfected cells. E Wound healing assay using miR-184 mimic/inhibitor-transfected hCEC. The wound space was measured at 2 h after scratching. F , G Measurement of the wound space area. F miR-184 mimic transfected cells showed reduced wound closure, while G no significant difference was observed in the miR-184 inhibitor transfected cells. H Schematic of sprouting angiogenesis assay using microfluidics chip. 20 ng/mL of VEGF gradient was generated to induce endothelial cell sprouting. I Sprouting angiogenesis assay using miR-184 mimic/inhibitor transfected hCEC. Cells were analyzed at 48 h after generating a VEGF gradient. J The number of tip cells per mm was measured and analyzed. miR-184 mimic transfected cells express significantly low tip cells. All assays were performed with three replicative cultures. Graph values are represented as mean ± standard deviation. Statistical significance indicated as *P < 0.05, **P < 0.01, (all n = 3), B , C Scale bar = 1 mm. E – I Scale bar = 20 μm

    Article Snippet: Microfluidic plastic chips and chip holders to maintain chip humidity were purchased from AIM Biotech company (AIM Biotech, Singapore).

    Techniques: Migration, Isolation, Magnetic Beads, Transfection, Tube Formation Assay, Wound Healing Assay, Angiogenesis Assay, Generated, Standard Deviation